Ahh Bea, they laugh at me at my new job when I poured my gel instead of using the precasted ones. then they realised that they never use the 1% gels and that we don't have a precasted one... and then they saw how fast it was for me
although, in general I like the pre cast ones since it makes it less messy (but if we don't have any, I can plan aroudnthe pouring the gel and keep myself occupied until it is ready to go).
I'm still stuck with the whole "if you know what the kit does, you can use it" but if you don't know Buffer A and buffer B, you need to learn... I was supposed to help trouble shooting the other week but got stuck with the first question "What did you do?" since the person started by saying "I added this buffer from the kit and then ran it".... not knowing really what or how it worked. That's when I feel ancient [but also knowledgable though, so I guess there is a positive side to it].
when working with bacteria I ended up mixing old and new protocols, for example there were this wonderful new tubes to "catch" chlorophorm and fenol when you spun them in a centrifuge. WONDERFUL. not only could I have larger quantities than 1.5 ml tubes with the c/f and therefore get less loss, I could minimise my own exposure to the smelly organics. They weren't too cheap though.... but boy, they sure made a place in my heart
"One never notices what has been done, one can only see what remains to be done" Marie Curie